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Histological Developmental Lens Regeneration of Rana cyanophlyctis

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Lens regeneration image 1 showing insertion of needle into the right eye of adult frog Rana cyanophlyctis for lentectomy







Lens regeneration image 2 Microphotograph of a section passing passing through dorsal iris (D1) of Emblica treated tadpole of R. cyanophlyctis showing depigementation of PECs, dorsal iris becomes swollen and knob like.











Lens regeneration image 3 Microphotograph of a section passing lentectomized eye of 3 days Emblica treated tadpole of R. cyanophlyctis showing cleft formation in between two laminae of dorsal iris.











Lens regeneration image 4 Microphotograph of a section passing passing through lentectomized eye of 5 days Emblica treated tadpole of R. cyanophlyctis showing formation of lens vesicle at the tip of dorsal iris.









Lens regeneration image 5 Microphotograph of a section passing passing through lentectomized eye of 5 days Emblica treated tadpole of R. cyanophlyctis showing formation of lens vesicle at the tip of dorsal iris.









Lens regeneration image 6 Microphotograph of a section passing passing through lentectomized eye of 5 days Emblica treated tadpole showing well defined lens vesicle.









Lens regeneration image 7 Microphotograph of a hand section passing through the operated eye showing regenerated lens attached with dorsal iris of 7 day Emblica treated tadpole of R. cyanophlyctis.









Lens regeneration image 8 Microphotograph of a section passing through the eye of 15 days Emblica treated froglet showing newly formed regenerated lens. Lens epithelium and differentiating lens fibers can be seen









Lens regeneration image 9 Microphotograph of a 40 days old Emblica treated tadpole of R. cyanophlyctis, showing well developed regenerated lens in the enucleated eye ball whose lens was extracted on day 0.









Lens regeneration image 10 Microphotograph Photograph of an operated tadpole ( lententomized) showing normal looking eye with regenerated lens.





Images©Indian J Exp Biol. 2009 Mar;47(3):157-62 (Developmental Biology Lab, MGS University Bikaner)

Descriptions-

[DI: Dorsal iris, DDC : Dedifferentiated cells, KLS : Knob like structure, LV : Lens vesicle, C : Cornea, RL : Regenerated lens, DLF : Differentiating lens fibers, R : Retina, EWRL : Eye with regenerated lens, CL : Cleft, LFC : Lens forming cells]